strong-arm mutagenesis is an effective mutational breeding method for improving various morphological characteristic of decorative plant . The experiment was undertaken to investigate the effect of various dose of gamma irradiation on semen sprouting of Catharanthus roseus ( Madagascar Cape periwinkle ) . The experiment was conducted at UCIARS , Weligatta to regain out the appropriate Vasco da Gamma dosages for selecting planting materials for C. roseus with the purpose of craw betterment under da Gamma ray of light . Healthy age dry seeds were exposed to 60 CO Gamma irradiation source at Horticultural Crops Research and Development Institute ( HORDI ) , Gannoruwa , Sri Lanka . Treatments included six dissimilar gamma dosages such as ( T0 - controller ) 0 Gy , ( T1 ) 100 Gy , ( T2 ) 200 Gy , ( T3 ) 300 Gy , ( T4 ) 400 Gy and ( T5 ) 500 Gy .
A Completely Randomized Design with four replicates and twelve samples per replicate was engage under shade planetary house precondition . Data on germination were collected day by day until constant sprouting was celebrate for up to 10 day , while other management practices were keep . data point were take apart using Minitab 17 statistical software with one - way ANOVA and Tukey ’s run to identify meaning differences among discussion at a 0.05 import level . The results revealed that the higher dosages of 60 CO Gamma actinotherapy significantly decreased the sprouting ability of C. roseus seeds ( P<0.05 ) . Specifically , 400 Gy and 500 Gy discussion result in important reductions in sprouting , whereas low dosage less than 400 Gy did not present such effects . germ germination started on day 3 after planting for all treatments except for T5 ( 500 Gy ) . point a delayed germination founding , seeds subjected to the T5 treatment lead off germination on day 5 after planting and demonstrate a significantly lower overall germination percentage when compared to other handling .
The gamey germination pace ( 84 % ) was observed in the control seeds , which were not subject to Vasco da Gamma irradiation , whereas the low germination rate ( 8 % ) occurred in seed treat with 500 Gy of da Gamma shaft . High State of Vasco da Gamma ray negatively impact germination by potentially induce oxidative stress , which go to cellular damage and foreshorten germination efficiency . At higher doses , such as 400 Gy and 500 Gy ( as in T4 and T5 treatments respectively ) radiation can cause significant damage to the deoxyribonucleic acid and cellular membrane , leading to check germination initiation and lose weight overall germination percentages . These findings have pragmatic implications for the utilization of da Gamma ray of light in plant life breeding and ejaculate preservation , emphasizing the penury to carefully optimize irradiation doses to avoid adverse effects on seed sprouting of C. roseus .
Sriwarnsinghe , Nelka & Vidanapthirana , Nisansala & Silva , Tara & Subasinghe , Siripala & Dahanayake , Nilanthi . ( 2024 ) . EFFECT OF GAMMA IRRADIATION ON SEED GERMINATION OF Catharanthus roseus ( MADAGASCAR PERIWINKLE ) .
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